Relationship between ulcer healing after hyperbaric oxygen therapy and transcutaneous oximetry, toe blood pressure and ankle-brachial index in patients with diabetes and chronic foot ulcers.
Abstract: The randomised, double-blind, placebo-controlled Hyperbaric Oxygen Therapy (HBOT) in Diabetic Patients with Chronic Foot Ulcers (HODFU) study showed beneficial effect of HBOT. As this treatment is expensive and time-consuming, being able to select patients...Mechanism of lysine oxidation in human lens crystallins during aging and in diabetes.
Oxidative mechanisms during nuclear sclerosis of the lens are poorly understood, in particular metal-catalyzed oxidation. The lysyl oxidation product adipic semialdehyde (allysine, ALL) and its oxidized end-product 2-aminoadipic acid (2-AAA) were determined as a function of age and presence of diabetes. Surprisingly, whereas both ALL and 2-AAA increased with age and strongly correlated with cataract grade and protein absorbance at 350 nm, only ALL formation but not 2-AAA was increased by diabetes. To clarify the mechanism of oxidation, rabbit lenses were treated with hyperbaric oxygen (HBO) for 48 h, and proteins were analyzed by gas and liquid chromatography mass spectrometry for ALL, 2-AAA, and multiple glycation products. Upon exposure to HBO, rabbit lenses were swollen, and nuclei were yellow. Protein-bound ALL increased 8-fold in the nuclear protein fractions versus controls. A dramatic increase in methyl-glyoxal hydroimidazolone and carboxyethyl-lysine but no increase of 2-AAA occurred, suggesting more drastic conditions are needed to oxidize ALL into 2-AAA. Indeed the latter formed only upon depletion of glutathione and was catalyzed by H(2)O(2).
Diabetes: foot ulcers and amputations.
Abstract: Diabetic foot ulceration is full-thickness penetration of the dermis of the foot in a person with diabetes. Severity is classified using the Wagner system, which grades it from 1 to 5. The annual incidence of ulcers among people with diabetes is 2.5-10.7% in...Clinical Trial – Bone Marrow Mononuclear Cell and Hyperbaric Oxygen Therapy in Diabetes Mellitus
There were evidences that the non-immune mediated inflammatory pathways of cell damage
occurred in vitro in human islets upon hyperglycemia in type 2 diabetes mellitus. Autologous
stem cell therapies were an emerging set of therapies that showed promise with a low side
effect profile. we hypothesized that infusion of mononuclear cells from buffy coat obtained
from bone marrow might provide multiple signals for regeneration and inflammation-induced
lesion recovery of local tissues, of which the effect might be maximized by intra-arterial
pancreatic infusion through angiography and combination with hyperbaric oxygen therapy.
This trail includes a foregoing sub-trial that investigate the feasibility and safety of a
novel method for massive bone marrow collection. The traditional BM collecting procedure is
unfavorable because it yields minor bone marrow. Studies have shown that physiological
exercise can increase bone marrow blood flow, which might facilitate BM collection.
We plan to include a total of 60 patients with type 2 diabetes and randomly assign them to
either a control group or an exercise group (n =30 each). The patients in the exercise group
exercised 30 minutes before the operation. All patients underwent routine surgical care. The
collected BM volume, operation time, collecting speed , puncture times and pain scores during
the operation were recorded. Bone marrow samples were tested for CD34+ flow cytometry and
whole blood cell count.